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Bioprinting-Based PDLSC-ECM Screening for in Vivo Repair of Alveolar Bone Defect Using Cell-Laden, Injectable and Photocrosslinkable Hydrogels EI SCIE Scopus
期刊论文 | 2017 , 3 (12) , 3534-3545 | ACS BIOMATERIALS SCIENCE & ENGINEERING | IF: 4.432
WoS CC Cited Count: 2
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Abstract :

Periodontitis is an inflammatory disease worldwide that may result in periodontal defect (especially alveolar bone defect) and even tooth loss. Stem-cell-based approach combined with injectable hydrogels has been proposed as a promising strategy in periodontal treatments. Stem cells fate closely depends on their extracellular matrix (ECM) characteristics. Hence, it is necessary to engineer an appropriate injectable hydrogel to deliver stem cells into the defect while serving as the ECM during healing. Therefore, stem cell-ECM interaction should be studied for better stem cell transplantation. In this study, we developed a bioprinting-based strategy to study stem cell-ECM interaction and thus screen an appropriate ECM for in vivo repair of alveolar bone defect. Periodontal ligament stem cells (PDLSCs) were encapsulated in injectable, photocrosslinkable composite hydrogels composed of gelatin methacrylate (GelMA) and poly(ethylene glycol) dimethacrylate (PEGDA). PDLSC-laden GelMA/PEGDA hydrogels with varying composition were efficiently fabricated via a 3D bioprinting platform by controlling the volume ratio of GelMA-to-PEGDA. PDLSC behavior and fate were found to be closely related to the engineered ECM composition. The 4/1 GelMA/PEGDA composite hydrogel was selected since the best performance in osteogenic differentiation in vitro. Finally, in vivo study indicated a maximal and robust new bone formation in the defects treated with the PDLSC-laden hydrogel with optimized composition as compared to the hydrogel alone and the saline ones. The developed approach would be useful for studying cell-ECM interaction in 3D and paving the way for regeneration of functional tissue.

Keyword :

cell-ECM screening injectable hydrogel 3D bioprinting PDLSCs alveolar bone repair

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GB/T 7714 Ma, Yufei , Ji, Yuan , Zhong, Tianyu et al. Bioprinting-Based PDLSC-ECM Screening for in Vivo Repair of Alveolar Bone Defect Using Cell-Laden, Injectable and Photocrosslinkable Hydrogels [J]. | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2017 , 3 (12) : 3534-3545 .
MLA Ma, Yufei et al. "Bioprinting-Based PDLSC-ECM Screening for in Vivo Repair of Alveolar Bone Defect Using Cell-Laden, Injectable and Photocrosslinkable Hydrogels" . | ACS BIOMATERIALS SCIENCE & ENGINEERING 3 . 12 (2017) : 3534-3545 .
APA Ma, Yufei , Ji, Yuan , Zhong, Tianyu , Wan, Wanting , Yang, Qingzhen , Li, Ang et al. Bioprinting-Based PDLSC-ECM Screening for in Vivo Repair of Alveolar Bone Defect Using Cell-Laden, Injectable and Photocrosslinkable Hydrogels . | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2017 , 3 (12) , 3534-3545 .
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3D Conformal Modification of Electrospun Silk Nanofibers with Nanoscaled ZnO Deposition for Enhanced Photocatalytic Activity EI SCIE Scopus
期刊论文 | 2017 , 3 (11) , 2900-2906 | ACS BIOMATERIALS SCIENCE & ENGINEERING | IF: 4.432
WoS CC Cited Count: 2 SCOPUS Cited Count: 3
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Abstract :

Nanostructured metal oxide materials have drawn great attention because of their enhanced semiconducting, electrical, optical, sensing, and chemical catalyzing properties. The application of metal oxides in biomedicine has recently emerged as a promising field, especially in the format of organic/metal oxide composites. However, the existing methods for fabricating organic/metal oxide materials revealed limitations on the precise control over the deposition of metal oxides and the maintenance of organic nanostructures. Here, we developed an approach for the fabrication of composite materials by depositing metal oxides on the nanostructured organic templates through atomic layer deposition (ALD). With this method, we fabricated a series of silk fibroin/ZnO composites at varied deposition temperatures. The results demonstrated that the ZnO layer had a 3D conformality and hexagonal wurtzite structure, and the deposition thickness was well controlled. The photocatalytic activity of silk/ZnO composites was confirmed by the photodegradation of Rh B under UV exposure, and the efficiency was found to be temperature dependent. These results demonstrated the successful integration of organic materials with metal oxides through an easy and controllable approach for the development of multifunctional organic/metal oxide biomaterials.

Keyword :

silk fibroin nanofibers atomic layer deposition (ALD) ZnO electrospun materials

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GB/T 7714 Zhao, Guoxu , Zhang, Yijun , Zhang, Le et al. 3D Conformal Modification of Electrospun Silk Nanofibers with Nanoscaled ZnO Deposition for Enhanced Photocatalytic Activity [J]. | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2017 , 3 (11) : 2900-2906 .
MLA Zhao, Guoxu et al. "3D Conformal Modification of Electrospun Silk Nanofibers with Nanoscaled ZnO Deposition for Enhanced Photocatalytic Activity" . | ACS BIOMATERIALS SCIENCE & ENGINEERING 3 . 11 (2017) : 2900-2906 .
APA Zhao, Guoxu , Zhang, Yijun , Zhang, Le , Ye, Zuo-Guang , Ren, Wei , Xu, Feng et al. 3D Conformal Modification of Electrospun Silk Nanofibers with Nanoscaled ZnO Deposition for Enhanced Photocatalytic Activity . | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2017 , 3 (11) , 2900-2906 .
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Engineering of microscale three-dimensional pancreatic islet models in vitro and their biomedical applications EI SCIE PubMed Scopus
期刊论文 | 2016 , 36 (4) , 619-629 | CRITICAL REVIEWS IN BIOTECHNOLOGY | IF: 6.542
WoS CC Cited Count: 5
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Abstract :

Diabetes now is the most common chronic disease in the world inducing heavy burden for the people's health. Based on this, diabetes research such as islet function has become a hot topic in medical institutes of the world. Today, in medical institutes, the conventional experiment platform in vitro is monolayer cell culture. However, with the development of micro- and nano-technologies, several microengineering methods have been developed to fabricate three-dimensional (3D) islet models in vitro which can better mimic the islet of pancreases in vivo. These in vitro islet models have shown better cell function than monolayer cells, indicating their great potential as better experimental platforms to elucidate islet behaviors under both physiological and pathological conditions, such as the molecular mechanisms of diabetes and clinical islet transplantation. In this review, we present the state-of-the-art advances in the microengineering methods for fabricating microscale islet models in vitro. We hope this will help researchers to better understand the progress in the engineering 3D islet models and their biomedical applications such as drug screening and islet transplantation.

Keyword :

three dimensional microengineering methods Cell microenvironment islet model

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GB/T 7714 Gao, Bin , Wang, Lin , Han, Shuang et al. Engineering of microscale three-dimensional pancreatic islet models in vitro and their biomedical applications [J]. | CRITICAL REVIEWS IN BIOTECHNOLOGY , 2016 , 36 (4) : 619-629 .
MLA Gao, Bin et al. "Engineering of microscale three-dimensional pancreatic islet models in vitro and their biomedical applications" . | CRITICAL REVIEWS IN BIOTECHNOLOGY 36 . 4 (2016) : 619-629 .
APA Gao, Bin , Wang, Lin , Han, Shuang , Pingguan-Murphy, Belinda , Zhang, Xiaohui , Xu, Feng . Engineering of microscale three-dimensional pancreatic islet models in vitro and their biomedical applications . | CRITICAL REVIEWS IN BIOTECHNOLOGY , 2016 , 36 (4) , 619-629 .
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Antiproliferative Activity and Cellular Uptake of Evodiamine and Rutaecarpine Based on 3D Tumor Models SCIE PubMed Scopus
期刊论文 | 2016 , 21 (7) | MOLECULES | IF: 2.861
WoS CC Cited Count: 4 SCOPUS Cited Count: 2
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Abstract :

Evodiamine (EVO) and rutaecarpine (RUT) are promising anti-tumor drug candidates. The evaluation of the anti-proliferative activity and cellular uptake of EVO and RUT in 3D multicellular spheroids of cancer cells would better recapitulate the native situation and thus better reflect an in vivo response to the treatment. Herein, we employed the 3D culture of MCF-7 and SMMC-7721 cells based on hanging drop method and evaluated the anti-proliferative activity and cellular uptake of EVO and RUT in 3D multicellular spheroids, and compared the results with those obtained from 2D monolayers. The drugs' IC50 values were significantly increased from the range of 6.4-44.1 M in 2D monolayers to 21.8-138.0 M in 3D multicellular spheroids, which may be due to enhanced mass barrier and reduced drug penetration in 3D models. The fluorescence of EVO and RUT was measured via fluorescence spectroscopy and the cellular uptake of both drugs was characterized in 2D tumor models. The results showed that the cellular uptake concentrations of RUT increased with increasing drug concentrations. However, the EVO concentrations uptaken by the cells showed only a small change with increasing drug concentrations, which may be due to the different solubility of EVO and Rut in solvents. Overall, this study provided a new vision of the anti-tumor activity of EVO and RUT via 3D multicellular spheroids and cellular uptake through the fluorescence of compounds.

Keyword :

auto-fluorescence 3D multicellular spheroids hanging drop method cellular uptake

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GB/T 7714 Guo, Hui , Liu, Dongmei , Gao, Bin et al. Antiproliferative Activity and Cellular Uptake of Evodiamine and Rutaecarpine Based on 3D Tumor Models [J]. | MOLECULES , 2016 , 21 (7) .
MLA Guo, Hui et al. "Antiproliferative Activity and Cellular Uptake of Evodiamine and Rutaecarpine Based on 3D Tumor Models" . | MOLECULES 21 . 7 (2016) .
APA Guo, Hui , Liu, Dongmei , Gao, Bin , Zhang, Xiaohui , You, Minli , Ren, Hui et al. Antiproliferative Activity and Cellular Uptake of Evodiamine and Rutaecarpine Based on 3D Tumor Models . | MOLECULES , 2016 , 21 (7) .
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Labeling and long-term tracking of bone marrow mesenchymal stem cells in vitro using NaYF4:Yb3+,Er3+ upconversion nanoparticles SCIE PubMed Scopus
期刊论文 | 2016 , 42 , 199-208 | ACTA BIOMATERIALIA | IF: 6.319
WoS CC Cited Count: 13
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Abstract :

Mesenchymal stem cells (MSCs) hold great promise as cell therapy candidate in clinics. However, the underlying mechanisms remain elusive due to the lack of effective cell tracking approaches during therapeutic processes. In this study, we successfully synthesized and utilized NaYF4:Yb3+,Er3+ upconversion nanoparticles (UCNPs) to label and track rabbit bone marrow mesenchymal stem cells (rBMSCs) during the osteogenic differentiation in vitro. To improve their biocompatibility and cellular uptake, we modified the UCNPs with negatively-charged poly(acrylic acid) and positively-charged poly(allylamine hydrochloride) in turns (i.e., PAH-PAA-UCNPs). The effect of cellular uptake of UCNPs on the osteogenic differentiation of rBMSCs was systematically evaluated, and no significant difference was found between rBMSCs labeled with UCNPs (concentration range of 0-50 mu g/mL) and UCNPs-free rBMSCs in terms of cell viability, ALP activity, osteogenic protein expressions and production of mineralized nodules. Moreover, the PAH-PAA-UCNPs at a concentration of 50 mu g/mL exhibited the highest biocompatibility and stability, which could well track rBMSCs during the osteogenesis process. These results would provide a positive reference for the application of these lanthanide-doped UCNPs as fluorescent nanoprobes for stem cell tracking to further understand the mechanism of stem cell fate in tissue engineering and stem cell therapy. Statement of Significance Upconversion nanoparticles (UCNPs) have attracted increasing attention as alternative probes for tracking various types of cells including stem cells. The reported fluorapatite-based UCNPs with the needle-like morphology showed a little poor performance on stem cell tracking, which was possibly attributed to the low upconversion efficiency and cell labeling efficiency potentially due to nanomaterial composition, crystal structure and shape. Here, we synthesized the positively-charged NaYF4:Yb3+,Er3+ UCNPs with hexagonal phase and sphere-like morphology to enhance their upconversion efficiency, biocompatibility and cellular uptake, leading to a successful tracking of rBMSCs in osteogenesis process without impairing cell viability and differentiation capacity. This study provided a necessary reference for the application of UCNPs in stem cell tracking to better understand the mechanism of stem cell fate in tissue engineering, stem cell therapy, etc. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Keyword :

Osteogenic differentiation Cell tracking Upconversion nanoparticles Mesenchymal stem cells

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GB/T 7714 Ma, Yufei , Ji, Yuan , You, Minli et al. Labeling and long-term tracking of bone marrow mesenchymal stem cells in vitro using NaYF4:Yb3+,Er3+ upconversion nanoparticles [J]. | ACTA BIOMATERIALIA , 2016 , 42 : 199-208 .
MLA Ma, Yufei et al. "Labeling and long-term tracking of bone marrow mesenchymal stem cells in vitro using NaYF4:Yb3+,Er3+ upconversion nanoparticles" . | ACTA BIOMATERIALIA 42 (2016) : 199-208 .
APA Ma, Yufei , Ji, Yuan , You, Minli , Wang, Shurui , Dong, Yuqing , Jin, Guorui et al. Labeling and long-term tracking of bone marrow mesenchymal stem cells in vitro using NaYF4:Yb3+,Er3+ upconversion nanoparticles . | ACTA BIOMATERIALIA , 2016 , 42 , 199-208 .
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Recent Advances in Electrospun Nanofibrous Scaffolds for Cardiac Tissue Engineering EI SCIE Scopus
期刊论文 | 2015 , 25 (36) , 5726-5738 | ADVANCED FUNCTIONAL MATERIALS | IF: 11.382
WoS CC Cited Count: 53
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Abstract :

Cardiovascular diseases remain the leading cause of human mortality worldwide. Some severe symptoms, including myocardial infarction and heart failure, are difficult to heal spontaneously or under systematic treatment due to the limited regenerative capacity of the native myocardium. Cardiac tissue engineering has emerged as a practical strategy to culture functional cardiac tissues and relieve the disorder in myocardium when implanted. In cardiac tissue engineering, the design of a scaffold is closely relevant to the function of the regenerated cardiac tissues. Nanofibrous materials fabricated by electrospinning have been developed as desirable scaffolds for tissue engineering applications because of the biomimicking structure of protein fibers in native extra cellular matrix. The versatilities of electrospinning on the polymer component, the fiber structure, and the functionalization with bioactive molecules have made the fabrication of nanofibrous scaffolds with suitable mechanical strength and biological properties for cardiac tissue engineering feasible. Here, an overview of recent advances in various electrospun scaffolds for engineering cardiac tissues, including the design of advanced electrospun scaffolds and the performance of the scaffolds in functional cardiac tissue regeneration, is provided with the aim to offer guidance in the innovation of novel electrospun scaffolds and methods for improving their potential for cardiac tissue engineering applications.

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GB/T 7714 Zhao, Guoxu , Zhang, Xiaohui , Lu, Tian Jian et al. Recent Advances in Electrospun Nanofibrous Scaffolds for Cardiac Tissue Engineering [J]. | ADVANCED FUNCTIONAL MATERIALS , 2015 , 25 (36) : 5726-5738 .
MLA Zhao, Guoxu et al. "Recent Advances in Electrospun Nanofibrous Scaffolds for Cardiac Tissue Engineering" . | ADVANCED FUNCTIONAL MATERIALS 25 . 36 (2015) : 5726-5738 .
APA Zhao, Guoxu , Zhang, Xiaohui , Lu, Tian Jian , Xu, Feng . Recent Advances in Electrospun Nanofibrous Scaffolds for Cardiac Tissue Engineering . | ADVANCED FUNCTIONAL MATERIALS , 2015 , 25 (36) , 5726-5738 .
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